The effect of autologous conditioned plasma on the treatment of focal chondral defects of the knee. An experimental study

Int J Immunopathol Pharmacol. Jan-Mar 2011;24(1 Suppl 2):117-24. doi: 10.1177/03946320110241S222.


The purpose of the present study was to evaluate the effect of local application of Autologous Conditioned Plasma (ACP) on the treatment of full-thickness cartilage injuries of the knee. The hypothesis of the study was that ACP can enhance healing response of injured cartilage. A full-thickness chondral lesion on the weight-bearing area of the medial femoral condyle was performed in 30 sheep. Animals were divided into 2 groups, according to postoperative treatment: in group 1, weekly injections of ACP for five times were performed; in group 2, lesions were left untreated. Animals were sacrificed at 3, 6 and 12 months after treatment. A histological evaluation was performed according to a modified O'Driscoll histological score. Comparison between groups for each time interval was performed with the Student's t-test. Significance was set at P<0.05. A post-hoc power analysis was performed according to the hypothesis of the study. Histological evaluation at 3 and 6 months showed that group 1 had significantly greater total score than group 2. At 12 months, no significant difference was observed between groups. In conclusion, local injections of ACP for treatment of full-thickness cartilage injuries did not produce hyaline cartilage. However, it promoted reparative response of the cartilage defect until 6 months after treatment.

MeSH terms

  • Animals
  • Bone Regeneration / physiology
  • Cartilage, Articular / injuries
  • Cartilage, Articular / pathology*
  • Cartilage, Articular / surgery
  • Chondrocytes / pathology
  • Data Interpretation, Statistical
  • Endpoint Determination
  • Hindlimb / pathology
  • Hindlimb / surgery
  • Intercellular Signaling Peptides and Proteins / therapeutic use*
  • Joints / injuries*
  • Joints / pathology
  • Platelet-Rich Plasma*
  • Prostheses and Implants
  • Sheep
  • Treatment Outcome


  • Intercellular Signaling Peptides and Proteins