Adenovirus-mediated transfer of tris-shRNAs induced apoptosis of nasopharyngeal carcinoma cell in vitro and in vivo

Cancer Lett. 2011 Oct 28;309(2):162-9. doi: 10.1016/j.canlet.2011.05.028. Epub 2011 Jun 12.

Abstract

RNA interference (RNAi) is an evolutionary conserved mechanism for specific gene silencing. There are currently numerous cancer therapy clinical trials based on RNAi technology. Using an adenoviral system as a delivery mediator of RNAi, we investigated the therapeutic effects of targeting three genes simultaneously in vitro and in vivo. In this study, we constructed an recombinant adenoviral shRNA expression system as Adv-pEGFP-shVEGF-shTERT-shBcl-xl for multi-genes silencing. Our results showed that the adenoviral vector can achieve above 90% of transfection efficiency and induced obvious apoptosis in CNE-2 cell both in vitro and in vivo compared with targeting the TERT alone or controlled group.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Apoptosis*
  • Blotting, Western
  • Carcinoma
  • Cell Line, Tumor
  • Flow Cytometry
  • Genetic Vectors
  • Humans
  • Indoles
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms / genetics
  • Nasopharyngeal Neoplasms / metabolism
  • Nasopharyngeal Neoplasms / pathology
  • Nasopharyngeal Neoplasms / therapy*
  • Oxindoles
  • Polymerase Chain Reaction
  • RNA Interference*
  • RNA, Small Interfering
  • Telomerase / genetics
  • Transfection
  • Vascular Endothelial Growth Factor A / genetics
  • Xenograft Model Antitumor Assays
  • bcl-X Protein / genetics

Substances

  • BCL2L1 protein, human
  • Indoles
  • Oxindoles
  • RNA, Small Interfering
  • Vascular Endothelial Growth Factor A
  • bcl-X Protein
  • 152ORB
  • TERT protein, human
  • Telomerase