Membrane proteins as 14-3-3 clients in functional regulation and intracellular transport

Physiology (Bethesda). 2011 Jun;26(3):181-91. doi: 10.1152/physiol.00042.2010.


14-3-3 proteins regulate the function and subcellular sorting of membrane proteins. Often, 14-3-3 binding to client proteins requires phosphorylation of the client, but the relevant kinase is unknown in most cases. We summarize current progress in identifying kinases that target membrane proteins with 14-3-3 binding sites and discuss the molecular mechanisms of 14-3-3 action. One of the kinases involved is Akt/PKB, which has recently been shown to activate the 14-3-3-dependent switch in a number of client membrane proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • 14-3-3 Proteins / chemistry
  • 14-3-3 Proteins / metabolism*
  • Animals
  • Binding Sites
  • Cell Membrane / metabolism*
  • Humans
  • Phosphorylation
  • Protein Conformation
  • Protein Interaction Domains and Motifs
  • Protein Transport
  • Proto-Oncogene Proteins c-akt / metabolism
  • Signal Transduction*


  • 14-3-3 Proteins
  • Proto-Oncogene Proteins c-akt