Characterization of 2-octenoyl-CoA carboxylase/reductase utilizing pteB from Streptomyce avermitilis

Biosci Biotechnol Biochem. 2011;75(6):1191-3. doi: 10.1271/bbb.110003. Epub 2011 Jun 13.

Abstract

The filipin biosynthetic gene cluster of Streptomyces avermitilis contains pteB, a homolog of crotonyl-CoA carboxylase/reductase. PteB was predicted to be 2-octenoyl-CoA carboxylase/reductase, supplying hexylmalonyl-CoA to filipin biosynthesis. Recombinant PteB displayed selective reductase activity toward 2-octenoyl-CoA while generating a broad range of alkylmalonyl-CoAs in the presence of bicarbonate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl Coenzyme A / metabolism*
  • Acyl-CoA Dehydrogenases / chemistry
  • Acyl-CoA Dehydrogenases / genetics
  • Acyl-CoA Dehydrogenases / metabolism*
  • Anti-Bacterial Agents / chemistry*
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Filipin* / biosynthesis
  • Multigene Family
  • Plasmids
  • Sequence Homology, Amino Acid
  • Spectrophotometry
  • Streptomyces / chemistry
  • Streptomyces / enzymology*
  • Streptomyces / genetics
  • Transformation, Bacterial

Substances

  • Acyl Coenzyme A
  • Anti-Bacterial Agents
  • octenoyl-coenzyme A
  • Filipin
  • crotonyl-coenzyme A
  • Acyl-CoA Dehydrogenases
  • acyl-CoA dehydrogenase (NADP+)