Monovalent, clickable, uncharged, water-soluble perylenediimide-cored dendrimers for target-specific fluorescent biolabeling

J Am Chem Soc. 2011 Jul 6;133(26):9964-7. doi: 10.1021/ja2009136. Epub 2011 Jun 14.

Abstract

Herein we report the synthesis of water-soluble polyglycerol-dendronized perylenediimides with a single reactive group that undergoes high-yielding click reactions. Single-molecule studies and target-specific biolabeling are reported, including the highly specific labeling of proteins on the surface of living bacterial and mammalian cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / metabolism
  • Click Chemistry*
  • Dendrimers / chemical synthesis
  • Dendrimers / chemistry*
  • Dendrimers / metabolism
  • Escherichia coli / cytology
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / metabolism
  • Glycerol / chemistry
  • HeLa Cells
  • Humans
  • Imides / chemistry*
  • Molecular Imaging
  • Perylene / analogs & derivatives*
  • Perylene / chemistry
  • Polymers / chemistry
  • Solubility
  • Staining and Labeling
  • Substrate Specificity
  • Water / chemistry*

Substances

  • Bacterial Proteins
  • Dendrimers
  • Fluorescent Dyes
  • Imides
  • Polymers
  • perylenediimide
  • Water
  • polyglycerol
  • Perylene
  • Glycerol