Differential responses to blood pressure and oxidative stress in streptozotocin-induced diabetic Wistar-Kyoto rats and spontaneously hypertensive rats: effects of antioxidant (honey) treatment

Int J Mol Sci. 2011;12(3):1888-907. doi: 10.3390/ijms12031888. Epub 2011 Mar 16.


Oxidative stress is implicated in the pathogenesis and/or complications of hypertension and/or diabetes mellitus. A combination of these disorders increases the risk of developing cardiovascular events. This study investigated the effects of streptozotocin (60 mg/kg; ip)-induced diabetes on blood pressure, oxidative stress and effects of honey on these parameters in the kidneys of streptozotocin-induced diabetic Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). Diabetic WKY and SHR were randomized into four groups and received distilled water (0.5 mL) and honey (1.0 g/kg) orally once daily for three weeks. Control SHR had reduced malondialdehyde (MDA) and increased systolic blood pressure (SBP), catalase (CAT) activity, and total antioxidant status (TAS). SBP, activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were elevated while TAS was reduced in diabetic WKY. In contrast, SBP, TAS, activities of GPx and GR were reduced in diabetic SHR. Antioxidant (honey) treatment further reduced SBP in diabetic SHR but not in diabetic WKY. It also increased TAS, GSH, reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio, activities of GPx and GR in diabetic SHR. These data suggest that differences in types, severity, and complications of diseases as well as strains may influence responses to blood pressure and oxidative stress.

Keywords: antioxidant enzymes; diabetes mellitus; hypertension; kidney; oxidative stress; spontaneously hypertensive rats; streptozotocin; tualang honey.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antioxidants / pharmacology*
  • Antioxidants / therapeutic use
  • Blood Glucose / analysis
  • Blood Pressure / drug effects*
  • Body Weight / drug effects
  • Diabetes Mellitus, Experimental / chemically induced
  • Diabetes Mellitus, Experimental / metabolism*
  • Diabetes Mellitus, Experimental / prevention & control
  • Glutathione / metabolism
  • Honey*
  • Hypertension / metabolism*
  • Hypertension / pathology
  • Hypertension / prevention & control
  • Kidney / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Oxidoreductases / metabolism
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY
  • Streptozocin / toxicity


  • Antioxidants
  • Blood Glucose
  • Malondialdehyde
  • Streptozocin
  • Oxidoreductases
  • Glutathione