Molecular-based detection of non-culturable and emerging campylobacteria in patients presenting with gastroenteritis

Epidemiol Infect. 2012 Apr;140(4):684-8. doi: 10.1017/S0950268811000859. Epub 2011 May 18.


From January 2009 to May 2010, 436 faecal samples from patients with diarrhoeal illness in Southern Ireland were identified as Campylobacter genus-positive by an automated multiplex PCR; however, 204 (46·8%) of these samples were culture-negative for campylobacters. A combination of Campylobacter-specific uniplex PCR and 16S rRNA sequencing confirmed the presence of Campylobacter DNA in 191 (93·6%) of the culture-negative samples. Species-specific PCR identified C. jejuni (50·7%) C. ureolyticus (41%) and C. coli (5·7%) as the most prevalent species while C. fetus, C. upsaliensis, C. hyointestinalis and C. lari accounted for 10% of culture-negative samples; mixed Campylobacter spp. were detected in 11% of samples. We conclude that non-culturable Campylobacter spp. are responsible for a considerable proportion of human enteritis and the true incidence of infection is likely to be significantly underestimated where conventional Campylobacter culture methods are used in isolation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Campylobacter / genetics*
  • Campylobacter Infections / diagnosis
  • Campylobacter Infections / microbiology*
  • Campylobacter coli / genetics
  • Campylobacter jejuni / genetics
  • Feces / microbiology
  • Gastroenteritis / diagnosis
  • Gastroenteritis / microbiology*
  • Humans
  • Polymerase Chain Reaction