Field evaluation of five rapid diagnostic tests for screening of HIV-1 infections in rural Rakai, Uganda

Int J STD AIDS. 2011 Jun;22(6):308-9. doi: 10.1258/ijsa.2009.009352.

Abstract

The performance characteristics of HIV rapid diagnostic tests (RDTs) vary by test and by population. We assessed five commercial RDTs in Uganda where all but one RDT (Determine; Abbott Laboratories, Germany) performed close to manufacturer's expectations. Determine had low specificity (85.2%, positive predictive value 67.3%) due to false-positive results with weak-positive bands. Properly trained staff, good quality control programmes and validation of RDTs with laboratories having confirmatory testing capacity may be warranted to assure accuracy in each setting.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • False Positive Reactions
  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV-1 / isolation & purification*
  • Humans
  • Point-of-Care Systems
  • Reagent Kits, Diagnostic*
  • Reproducibility of Results
  • Rural Population
  • Sensitivity and Specificity
  • Uganda

Substances

  • Reagent Kits, Diagnostic