Solution NMR study of integral membrane proteins

Curr Opin Chem Biol. 2011 Aug;15(4):560-9. doi: 10.1016/j.cbpa.2011.05.025.


Signals between a cell and its environment are often transmitted through membrane proteins; therefore, many membrane proteins, including G protein-coupled receptors (GPCRs) and ion channels, are important drug targets. Structural information about membrane proteins remains limited owing to challenges in protein expression, purification and the selection of membrane-mimicking systems that will retain protein structure and function. This review describes recent advances in solution NMR applied to the structural study of integral membrane proteins. The examples herein demonstrate that solution NMR spectroscopy will play a unique role not only in structural analysis, but also drug discovery of membrane proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Detergents / chemistry*
  • Drug Discovery
  • Humans
  • Isotope Labeling
  • Lipid Bilayers / chemistry*
  • Magnetic Resonance Spectroscopy / methods
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Micelles
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Radioisotopes / chemistry*
  • Solutions
  • Structure-Activity Relationship


  • Detergents
  • Lipid Bilayers
  • Membrane Proteins
  • Micelles
  • Radioisotopes
  • Solutions