MicroRNA29a regulates the expression of the nuclear oncogene Ski

Blood. 2011 Aug 18;118(7):1899-902. doi: 10.1182/blood-2010-09-306258. Epub 2011 Jun 17.


MicroRNAs (miRNAs) are small, noncoding RNA molecules that regulate growth and differentiation. miRNAs are frequently located at cancer-specific fragile sites in the human genome, such as chromosome 7q. The nuclear oncogene SKI is up-regulated in acute myeloid leukemia (AML) with -7/del7q. Here we asked whether loss of miRNAs on chromosome 7q may explain this up-regulation. miR-29a expression was found to be down-regulated in AML with -7/del7q. Forced expression of miR-29a down-regulated Ski and its target gene, Nr-CAM, whereas miR-29a inhibition induced Ski expression. Luciferase assays validated a functional binding site for miR-29a in the 3' untranslated region of SKI. Finally, in samples of AML patients, we observed an inverse correlation of Ski and miR-29a expression, respectively. In conclusion, up-regulation of Ski in AML with -7/del7q is caused by loss of miR-29a. miR-29a may therefore function as an important tumor suppressor in AML by restraining expression of the SKI oncogene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 7
  • DNA-Binding Proteins / genetics*
  • Down-Regulation
  • Gene Expression Regulation, Leukemic*
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • MicroRNAs / genetics*
  • Proto-Oncogene Proteins / genetics*
  • Up-Regulation


  • 3' Untranslated Regions
  • DNA-Binding Proteins
  • MIRN29a microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins
  • SKI protein, human