Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

Wen Wang; Daniel I C Wang; Zhi Li

doi:10.1039/c1cc12685g

Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle

Chem Commun (Camb). 2011 Jul 28;47(28):8115-7. doi: 10.1039/c1cc12685g. Epub 2011 Jun 20.

Authors

Wen Wang¹, Daniel I C Wang, Zhi Li

Affiliation

¹ Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576.

PMID: 21687876
DOI: 10.1039/c1cc12685g

Abstract

Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis*
Enzymes, Immobilized / chemistry*
Enzymes, Immobilized / isolation & purification
Enzymes, Immobilized / metabolism*
Epoxide Hydrolases / chemistry
Epoxide Hydrolases / isolation & purification
Epoxide Hydrolases / metabolism
Magnetics*
Models, Molecular
Nanoparticles / chemistry*
Nickel / chemistry*
Nitrilotriacetic Acid / chemistry*
Protein Conformation
Solanum tuberosum / enzymology

Substances

Enzymes, Immobilized
Nickel
Epoxide Hydrolases
Nitrilotriacetic Acid