Wide-field imaging of fluorescent deoxy-glucose in ex vivo malignant and normal breast tissue

Biomed Opt Express. 2011 Jun 1;2(6):1514-23. doi: 10.1364/BOE.2.001514. Epub 2011 May 11.


Rapid in situ determination of surgical resection margins during breast cancer surgery would reduce patient time under anesthesia. We present preliminary data supporting the use of a fluorescent glucose analog (2-NBDG) as an optical contrast agent to differentiate freshly excised breast tissue containing cancerous cells from normal breast tissue. Multi-spectral images of 14 breast cancer specimens acquired before and after incubation with 2-NBDG demonstrated increased fluorescent signal in all of the malignant tissue due to increased 2-NBDG consumption. We demonstrate that 2-NBDG has potential as an optical contrast agent to differentiate cancerous from non-cancerous tissue.

Keywords: (170.1610) Clinical applications; (170.3880) Medical and biological imaging.