Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry

Org Lett. 2011 Aug 5;13(15):3770-3. doi: 10.1021/ol201404v. Epub 2011 Jun 24.


Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and organic substrates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acids / chemistry
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Molecular Structure
  • Peptides / analysis*
  • Peptides / chemistry


  • Amino Acids
  • Peptides