Purification of the tyrosine inhibitable 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Schizosaccharomyces pombe

M Bracher; E Schweingruber

doi:10.1016/0005-2744(77)90180-2

Purification of the tyrosine inhibitable 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase from Schizosaccharomyces pombe

Biochim Biophys Acta. 1977 Dec 8;485(2):446-51. doi: 10.1016/0005-2744(77)90180-2.

Authors

M Bracher, E Schweingruber

PMID: 21700
DOI: 10.1016/0005-2744(77)90180-2

Abstract

A method is described for the purification of the tyrosine inhibitable isoenzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase(pyruvate phosphorylating), EC 4.1.2.15) to homogeneity as judged by polyacrylamide gel electrophoresis.

MeSH terms

3-Deoxy-7-Phosphoheptulonate Synthase / antagonists & inhibitors
3-Deoxy-7-Phosphoheptulonate Synthase / isolation & purification*
Aldehyde-Lyases / isolation & purification*
Ascomycota / enzymology*
Molecular Weight
Schizosaccharomyces / enzymology*
Tyrosine / pharmacology*

Substances

Tyrosine
3-Deoxy-7-Phosphoheptulonate Synthase
Aldehyde-Lyases