Abstract
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caenorhabditis / genetics*
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Caenorhabditis elegans / genetics*
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Deoxyribonucleases, Type II Site-Specific / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism*
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Gene Targeting
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Genes, Helminth
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Genetic Techniques*
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Genome, Helminth*
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INDEL Mutation
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Mutagenesis
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Regulatory Elements, Transcriptional / genetics*
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Transcription Factors / chemistry
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Transgenes
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Zinc Fingers*
Substances
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Transcription Factors
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endodeoxyribonuclease FokI
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Deoxyribonucleases, Type II Site-Specific