Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae

Roger Dumke; Enno Jacobs

doi:10.1016/j.mimet.2011.06.008

Culture-independent multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae

J Microbiol Methods. 2011 Sep;86(3):393-6. doi: 10.1016/j.mimet.2011.06.008. Epub 2011 Jun 17.

Authors

Roger Dumke¹, Enno Jacobs

Affiliation

¹ Dresden University of Technology, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D, 01307 Dresden, Germany. roger.dumke@tu-dresden.de

PMID: 21704086
DOI: 10.1016/j.mimet.2011.06.008

Abstract

A PCR approach for multi-locus variable-number tandem-repeat analysis (MLVA) of Mycoplasma pneumoniae directly from respiratory samples was developed and evaluated on 54 specimens from M. pneumoniae-positive pneumonia patients. The method resulted in a clearly identifiable MLVA type in all samples tested and can be used for MLVA without laborious isolation of the pathogen.

MeSH terms

DNA, Bacterial / analysis
DNA, Bacterial / genetics
Humans
Minisatellite Repeats / genetics*
Molecular Diagnostic Techniques / methods*
Molecular Typing / methods
Mycoplasma pneumoniae / genetics*
Mycoplasma pneumoniae / isolation & purification*
Mycoplasma pneumoniae / metabolism
Pneumonia, Mycoplasma / diagnosis
Polymerase Chain Reaction / methods*

Substances

DNA, Bacterial