Targeting chromatin remodelers: signals and search mechanisms

Biochim Biophys Acta. 2011 Sep;1809(9):497-508. doi: 10.1016/j.bbagrm.2011.06.005. Epub 2011 Jun 16.

Abstract

Chromatin remodeling complexes are ATP-driven molecular machines that change chromatin structure by translocating nucleosomes along the DNA, evicting nucleosomes, or changing the nucleosomal histone composition. They are highly abundant in the cell and numerous different complexes exist that display distinct activity patterns. Here we review chromatin-associated signals that are recognized by remodelers. It is discussed how these regulate the remodeling reaction via changing the nucleosome substrate/product binding affinity or the catalytic translocation rate. Finally, we address the question of how chromatin remodelers operate in the cell nucleus to find specifically marked nucleosome substrates via a diffusion driven target location mechanism, and estimate the search times of this process. This article is part of a Special Issue entitled:Snf2/Swi2 ATPase structure and function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / metabolism*
  • Chromatin Assembly and Disassembly*
  • DNA / chemistry
  • DNA / metabolism
  • DNA Methylation
  • DNA-Binding Proteins / metabolism
  • Histones / metabolism
  • Nucleosomes / metabolism*
  • Protein Binding
  • Signal Transduction*
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism
  • Translocation, Genetic

Substances

  • DNA-Binding Proteins
  • Histones
  • Nucleosomes
  • Transcription Factors
  • Adenosine Triphosphate
  • DNA
  • Adenosine Triphosphatases