A Public Genome-Scale Lentiviral Expression Library of Human ORFs

Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638.

Abstract

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular / methods*
  • Genetic Vectors / genetics*
  • Genomic Library*
  • Humans
  • Lentivirus / genetics*
  • Open Reading Frames