Biomarkers of disease and post-mortem changes - Heat stabilization, a necessary tool for measurement of protein regulation

J Proteomics. 2011 Dec 10;75(1):145-59. doi: 10.1016/j.jprot.2011.06.009. Epub 2011 Jun 17.


This review focuses on post sampling changes and how the Stabilizor system has been used to control this natural biological process and potential implications on cancer-specific biomarkers due to post sampling changes. Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level [1] resulting in loss of sample quality due to post-mortem changes. A heat-stabilization technology, using the Stabilizor system, has been developed to quickly and permanently abolish the enzymatic activity that causes these changes post-sampling and so preserve sample quality. The Stabilizor system has been shown to give better sample quality when analyzing a variety of tissues in various proteomic workflows. In this paper we discuss the impact of using heat-stabilized tissue in different proteomic applications. Based on our observations regarding the overlap between commonly changing proteins and proteins found to change post-mortem we also highlight a group of proteins of particular interest in cancer studies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biomarkers / analysis*
  • Biomarkers / chemistry
  • Biomarkers / metabolism
  • Disease* / classification
  • Hot Temperature*
  • Humans
  • Postmortem Changes*
  • Protein Stability
  • Proteins / analysis*
  • Proteins / chemistry
  • Proteins / metabolism
  • Proteomics / methods*


  • Biomarkers
  • Proteins