Molecular characterization of Der p 10: a diagnostic marker for broad sensitization in house dust mite allergy

Clin Exp Allergy. 2011 Oct;41(10):1468-77. doi: 10.1111/j.1365-2222.2011.03798.x. Epub 2011 Jun 29.


Background: Tropomyosins represent clinically relevant seafood allergens but the role of mite tropomyosin, Der p 10, in house dust mite (HDM) allergy has not been studied in detail.

Objective: To express and purify a recombinant Der p 10 with equivalent IgE reactivity as natural Der p 10 and to evaluate its IgE reactivity and allergenic activity in HDM-allergic patients.

Methods: rDer p 10 was expressed in Escherichia coli, purified and characterized by mass spectrometry and circular dichroism. It was tested for IgE reactivity in 1322 HDM-allergic patients. Detailed IgE-reactivity profiles to six HDM allergens (Der p 1, 2, 5, 7, 10, 21) were established for subgroups of Der p 10-positive and -negative patients. The allergenic activity of rDer p 10 was evaluated in basophil degranulation experiments.

Results: rDer p 10 is an α-helical protein sharing IgE epitopes with nDer p 10. It is recognized by 15.2% of HDM-allergic patients. Der p 10-negative patients were primarily sensitized to Der p 1 and/or Der p 2, whereas Der p 10-positive patients reacted to several other HDM allergens besides the major allergens (Der p 1, Der p 2) or showed a rather selective Der p 10 reactivity. The allergenic activity of Der p 10 was generally low but patients could be identified who suffered from clinically relevant HDM allergy due to Der p 10 sensitization.

Conclusion and clinical relevance: Der p 10 may be a diagnostic marker for HDM-allergic patients with additional sensitization to allergens other than Der p 1 and Der p 2. Such patients may require attention when allergen-specific immunotherapy is considered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antigens, Dermatophagoides / chemistry
  • Antigens, Dermatophagoides / genetics*
  • Arthropod Proteins / chemistry
  • Arthropod Proteins / genetics*
  • Child
  • Circular Dichroism
  • Dermatophagoides pteronyssinus / immunology
  • Dermatophagoides pteronyssinus / metabolism
  • Desensitization, Immunologic / methods
  • Dust / immunology
  • Female
  • Humans
  • Hypersensitivity, Immediate / diagnosis*
  • Hypersensitivity, Immediate / immunology
  • Immunoglobulin E / blood
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Rats
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / isolation & purification
  • Tropomyosin / chemistry
  • Tropomyosin / genetics*
  • Young Adult


  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Dust
  • Recombinant Proteins
  • Tropomyosin
  • der p 10 protein, Dermatophagoides pteronyssinus
  • Immunoglobulin E