H-2 alleles contribute to antigen 85-specific interferon-gamma responses during Mycobacterium tuberculosis infection

Cell Immunol. 2011;271(1):53-61. doi: 10.1016/j.cellimm.2011.06.002. Epub 2011 Jun 12.


The in vitro immune responses to mycobacterial antigens have been linked to the H-2 loci in mice. We evaluated in vitro and in vivo immune responses during early Mycobacterium tuberculosis (M.tb) pulmonary infection of C57BL/6 (H-2(b)), C57BL/6 (H-2(k)), CBA/J (H-2(k)), and C3H/HeJ (H-2(k)) mice to determine H-2(k)-dependent and -independent effects. H-2(k)-dependent effects included delayed and diminished Ag85-specific Th1 cell priming, a reduced frequency of Ag85-specific IFN-γ producing cells, reduced IFN-γ protein in vivo, and increased M.tb lung burden as demonstrated by C57BL/6 H-2(k) mice vs. C57BL/6 mice. H-2(k)-independent factors controlled the amount of Ag85-specific IFN-γ produced by each cell, T cell numbers, granuloma size, and lymphocytic infiltrates in the lungs. Overall, these results suggest that an H-2(k)-dependent suboptimal generation of Ag85-specific cells impairs control of early M.tb growth in the lungs. H-2(k)-independent factors influence the potency of IFN-γ producing cells and immune cell trafficking during pulmonary M.tb infection.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Acyltransferases / immunology*
  • Alleles
  • Animals
  • Antigens, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • H-2 Antigens / genetics
  • H-2 Antigens / immunology*
  • Haplotypes
  • Host-Pathogen Interactions / immunology
  • Interferon-gamma / immunology*
  • Interferon-gamma / metabolism
  • Lung / immunology
  • Lung / metabolism
  • Lung / microbiology
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mycobacterium tuberculosis / immunology*
  • Mycobacterium tuberculosis / physiology
  • Tuberculosis, Pulmonary / immunology*
  • Tuberculosis, Pulmonary / metabolism
  • Tuberculosis, Pulmonary / microbiology


  • Antigens, Bacterial
  • H-2 Antigens
  • Interferon-gamma
  • Acyltransferases
  • antigen 85A, Mycobacterium tuberculosis