Development of allele-specific therapeutic siRNA for keratin 5 mutations in epidermolysis bullosa simplex

J Invest Dermatol. 2011 Oct;131(10):2079-86. doi: 10.1038/jid.2011.169. Epub 2011 Jun 30.


Epidermolysis bullosa simplex (EBS) is an incurable, inherited skin-blistering disorder predominantly caused by dominant-negative mutations in the genes encoding keratins K5 or K14. RNA interference, particularly in the form of small interfering RNA (siRNA), offers a potential therapy route for EBS and related keratin disorders by selectively silencing the mutant allele. Here, using a systemic screening system based on a luciferase reporter gene assay, we have developed mutant-specific siRNAs for two independent EBS-causing missense mutations in the K5 gene (p.Ser181Pro and p.Asn193Lys). The specificity of the allele-specific inhibitors identified in the screen was subsequently confirmed at the protein level, where the lead inhibitors were shown to strongly knock down the expression of mutant proteins with negligible effect on wild-type K5 expression. In a cell-based model system, the lead inhibitors were able to significantly reverse the cytoskeletal aggregation phenotype. Overall, this approach shows promise for the treatment of EBS and paves the way for future clinical trials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Dipodomys
  • Epidermolysis Bullosa Simplex / genetics*
  • Epidermolysis Bullosa Simplex / therapy*
  • Gene Silencing
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Keratin-14 / genetics
  • Keratin-5 / genetics*
  • Mutation*
  • Phenotype
  • RNA Interference
  • RNA, Small Interfering / metabolism*
  • Transfection


  • Keratin-14
  • Keratin-5
  • RNA, Small Interfering
  • Green Fluorescent Proteins