Protective effects on myelosuppression mice treated by three different classic Chinese medicine formulae

Pharmacogn Mag. 2011 Apr;7(26):133-40. doi: 10.4103/0973-1296.80671.

Abstract

Background: In order to observe the protective therapeutic action and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction, and Compound Danshen Decoction on Myelosuppression induced by cyclophosphamide.

Materials and methods: The mice model was established by intraperitoneal injected with 100 mg/kg cyclophosphamide by human and mice dose conversion on the 9(th), 11(th), 13(th) days during the experiment. Flow cytometry (FCM) was used for detecting the number of cells and investigating bone marrow cell cycles. Spleen was taken out and the mRNA expression level of thrombopoietin (TPO) and c-Mpl were detected by Q-PCR, and c-Mpl in spleen in order to discuss the mechanism of myelosuppression and the protective effects of traditional Chinese medicine.

Results: Both Liuwei Dihuang Decoction Group and Buzhong Yiqi Decoction Group can accelerate bone marrow hematopoietic stem progenitor cells (HSPCs) in marrow-suppressed mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S, G2/M phase. And at the same time these Chinese decoctions can increase the mRNA expression level of TPO and c-Mpl in spleen.

Conclusion: Researched showed that Chinese formula take effect by affecting these genes on myelosuppressed mice.

Keywords: Buzhong Yiqi decoction; Liuwei Dihuang decoction; cell cycle; compound Danshen decoction; myelosuppression; thrombopoietin; thrombopoietin receptor.