Dimethylformamide as a Cryoprotectant for Canine Semen Diluted and Frozen in ACP-106C

Theriogenology. 2011 Oct 15;76(7):1367-72. doi: 10.1016/j.theriogenology.2011.05.010. Epub 2011 Jun 30.

Abstract

The objective was to assess the effect of adding various concentrations of dimethylformamide on characteristics of canine semen diluted in powdered coconut water (ACP-106C; ACP Biotecnologia, Fortaleza, CE, Brazil) and frozen at -196°C. Fifteen ejaculates were collected by manual stimulation from five adult Boxer dogs. The sperm-rich fraction was diluted in ACP-106C (ACP Biotecnologia) containing 10% egg yolk and divided into four aliquots. The cryoprotectants used for each aliquot were 6% glycerol (control group; CG) or 2%, 4%, or 6% dimethylformamide (DF2, DF4, and DF6, respectively). After thawing, total motility (mean ± SEM) for CG (58.4 ± 24.6) was higher (P < 0.05) than that of the other groups (2% dimethylformamide, 24.4 ± 12.3; 4% dimethylformamide, 26.5 ± 16.1; and 6% dimethylformamide, 21.7 ± 17.9). Furthermore, there was a greater percentage of fast, average, and slow moving sperm (assessed with computer-aided semen analysis; CASA) in CG in comparison with the other three groups. Therefore, based on concentrations tested in this study, dimethylformamide, together with ACP-106C (ACP Biotecnologia) and 10% egg yolk as a diluent, yielded unsatisfactory in vitro results for freezing canine semen.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cocos / chemistry
  • Cryopreservation / methods
  • Cryopreservation / veterinary
  • Cryoprotective Agents / administration & dosage
  • Cryoprotective Agents / pharmacology*
  • Dimethylformamide / administration & dosage
  • Dimethylformamide / pharmacology*
  • Dogs
  • Male
  • Plant Preparations / administration & dosage
  • Plant Preparations / pharmacology*
  • Semen Preservation / methods
  • Semen Preservation / veterinary*
  • Semen*

Substances

  • ACP-106C
  • Cryoprotective Agents
  • Plant Preparations
  • Dimethylformamide