Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli from Rio de Janeiro, Brazil

Clin Microbiol Infect. 2011 Jul;17(7):1039-43. doi: 10.1111/j.1469-0691.2010.03440.x. Epub 2011 Jan 28.

Abstract

Twenty-five extended-spectrum β-lactamase (ESBL)-producing Escherichia coli clinical isolates from Rio de Janeiro, Brazil were characterized by isoelectric focusing, PCR and sequencing of bla(ESBL) genes, plasmid-mediated quinolone resistance determinants, phylogenetic groups, replicon typing, pulsed-field electrophoresis, and multilocus sequencing typing. Twenty-three (92%) ESBL-producing E. coli isolates were positive for bla(CTX-M) genes, aac(6')-Ib-cr, and qnrB. Genetic relatedness of ESBL producers clustered seven (28%) CTX-M-15-producing isolates as sequence type (ST)410, clonal complex (CC) 23, and two (8%) as clone O25-ST131. Our results illustrate the predominance of phylogroup A (52%), ST410 (CC 23) and CTX-M-15 among ESBL-producing E. coli isolates from hospitals in Rio de Janeiro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Electrophoresis, Gel, Pulsed-Field
  • Escherichia coli / classification
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology
  • Genotype
  • Humans
  • Isoelectric Focusing
  • Multilocus Sequence Typing
  • Plasmids
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / chemistry
  • beta-Lactamases / genetics*
  • beta-Lactamases / isolation & purification

Substances

  • DNA, Bacterial
  • beta-Lactamases