[Effects of CD74 gene on IFNγR gene expression in MG TEC]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2011 Jul;27(7):730-2.
[Article in Chinese]

Abstract

Aim: To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell(TEC).

Methods: PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR.

Results: Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells.

Conclusion: CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.

MeSH terms

  • Antigens, Differentiation, B-Lymphocyte / genetics*
  • Epithelial Cells / metabolism*
  • Gene Expression Regulation / genetics*
  • Histocompatibility Antigens Class II / genetics*
  • Humans
  • Immunomodulation / genetics
  • Male
  • Myasthenia Gravis / genetics*
  • Myasthenia Gravis / immunology
  • Plasmids / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Interferon / genetics*
  • Thymus Gland / pathology*
  • Transfection
  • Young Adult

Substances

  • Antigens, Differentiation, B-Lymphocyte
  • Histocompatibility Antigens Class II
  • RNA, Messenger
  • Receptors, Interferon
  • invariant chain
  • interferon gamma receptor