Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors

M R Shalaby; A Sundan; H Loetscher; M Brockhaus; W Lesslauer; T Espevik

doi:10.1084/jem.172.5.1517

Binding and regulation of cellular functions by monoclonal antibodies against human tumor necrosis factor receptors

J Exp Med. 1990 Nov 1;172(5):1517-20. doi: 10.1084/jem.172.5.1517.

Authors

M R Shalaby¹, A Sundan, H Loetscher, M Brockhaus, W Lesslauer, T Espevik

Affiliation

¹ Institute of Cancer Research, University of Trondheim, Norway.

Abstract

The present study was undertaken to further characterize the interaction of monoclonal antibodies (mAbs) against tumor necrosis factor (TNF) receptors with different targets, and to assess their ability to influence TNF effects on U937 and human endothelial cell (HEC) functions. Actions of recombinant TNF-alpha on U937 and HEC were effectively inhibited by Htr-5 and Utr-1, and to a greater extent by a combination of both mAbs. These observations indicate that TNF interaction with antigenically different components of membrane receptors (p55 and p75) represents a crucial step in transduction of signals for TNF toxicity against U937 and TNF activation of HEC functions.

MeSH terms

Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / physiology*
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism
Endothelium, Vascular / ultrastructure*
Humans
Leukemia, Myeloid / metabolism
Leukemia, Myeloid / pathology
Receptors, Cell Surface / drug effects
Receptors, Cell Surface / immunology*
Receptors, Cell Surface / metabolism
Receptors, Tumor Necrosis Factor
Tumor Cells, Cultured / drug effects
Tumor Cells, Cultured / pathology
Tumor Cells, Cultured / ultrastructure
Tumor Necrosis Factor-alpha / metabolism
Tumor Necrosis Factor-alpha / toxicity

Substances

Antibodies, Monoclonal
Receptors, Cell Surface
Receptors, Tumor Necrosis Factor
Tumor Necrosis Factor-alpha