Identification of bacterial target proteins for the salicylidene acylhydrazide class of virulence-blocking compounds

J Biol Chem. 2011 Aug 26;286(34):29922-31. doi: 10.1074/jbc.M111.233858. Epub 2011 Jul 1.


A class of anti-virulence compounds, the salicylidene acylhydrazides, has been widely reported to block the function of the type three secretion system of several Gram-negative pathogens by a previously unknown mechanism. In this work we provide the first identification of bacterial proteins that are targeted by this group of compounds. We provide evidence that their mode of action is likely to result from a synergistic effect arising from a perturbation of the function of several conserved proteins. We also examine the contribution of selected target proteins to the pathogenicity of Yersinia pseudotuberculosis and to expression of virulence genes in Escherichia coli O157.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology*
  • Escherichia coli Infections / drug therapy
  • Escherichia coli O157* / metabolism
  • Escherichia coli O157* / pathogenicity
  • Gene Expression Regulation, Bacterial / drug effects*
  • Hydrazines / chemistry
  • Hydrazines / pharmacology*
  • Virulence Factors / biosynthesis*
  • Yersinia pseudotuberculosis Infections / drug therapy
  • Yersinia pseudotuberculosis* / metabolism
  • Yersinia pseudotuberculosis* / pathogenicity


  • Anti-Bacterial Agents
  • Hydrazines
  • Virulence Factors

Associated data

  • GEO/GSE23001