Megapixel digital PCR

Nat Methods. 2011 Jul 3;8(8):649-51. doi: 10.1038/nmeth.1640.


We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Mutational Analysis / instrumentation*
  • Equipment Design
  • Gene Expression Profiling / instrumentation*
  • Microfluidics / instrumentation*
  • Polymerase Chain Reaction / instrumentation*