Protective effect of hydrogen gas therapy after germinal matrix hemorrhage in neonatal rats

Acta Neurochir Suppl. 2011;111:237-41. doi: 10.1007/978-3-7091-0693-8_40.


Background: Germinal matrix hemorrhage (GMH) is a neurological disease of very low birth weight premature infants leading to post-hemorrhagic hydrocephalus, cerebral palsy, and mental retardation. Hydrogen (H2) is a potent antioxidant shown to selectively reverse cytotoxic oxygen-radical injury in the brain. This study investigated the therapeutic effect of hydrogen gas after neonatal GMH injury.

Methods: Neonatal rats underwent stereotaxic infusion of clostridial collagenase into the right germinal matrix brain region. Cognitive function was assessed at 3 weeks, and then sensorimotor function, cerebral, cardiac and splenic growths were measured 1 week thereafter.

Results: Hydrogen gas inhalation markedly suppressed mental retardation and cerebral palsy outcomes in rats at the juvenile developmental stage. The administration of H2 gas, early after neonatal GMH, also normalized the brain atrophy, splenomegaly and cardiac hypertrophy 1 month after injury.

Conclusion: This study supports the role of cytotoxic oxygen-radical injury in early neonatal GMH. Hydrogen gas inhalation is an effective strategy to help protect the infant brain from the post-hemorrhagic consequences of brain atrophy, mental retardation and cerebral palsy. Further studies are necessary to determine the mechanistic basis of these protective effects.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Cerebral Hemorrhage / chemically induced*
  • Cerebral Hemorrhage / complications
  • Cerebral Hemorrhage / prevention & control*
  • Cognition Disorders / drug therapy
  • Cognition Disorders / etiology
  • Collagenases / toxicity*
  • Disease Models, Animal
  • Functional Laterality / drug effects
  • Gases / administration & dosage*
  • Hydrogen / administration & dosage*
  • Nervous System Diseases / drug therapy
  • Nervous System Diseases / etiology
  • Neuroprotective Agents / administration & dosage*
  • Organ Size / drug effects
  • Posture
  • Psychomotor Performance / drug effects
  • Rats
  • Reaction Time / drug effects
  • Reflex / drug effects
  • Somatosensory Disorders / drug therapy
  • Somatosensory Disorders / etiology


  • Gases
  • Neuroprotective Agents
  • Hydrogen
  • Collagenases