Multiple subtypes of serotonin receptors in the feeding circuit of a pond snail

Zoolog Sci. 2011 Jul;28(7):517-25. doi: 10.2108/zsj.28.517.

Abstract

In the central nervous system of the pond snail Lymnaea stagnalis, serotonergic transmission plays an important role in controlling feeding behavior. Recent electrophysiological studies have claimed that only metabotropic serotonin (5-HT(2)) receptors, and not ionotropic (5-HT(3)) receptors, are used in synapses between serotonergic neurons (the cerebral giant cells, CGCs) and the follower buccal motoneurons (the B1 cells). However, these data are inconsistent with previous results. In the present study, we therefore reexamined the serotonin receptors to identify the receptor subtypes functioning in the synapses between the CGCs and the B1 cells by recording the compound excitatory postsynaptic potential (EPSP) of the B1 cells evoked by a train of stimulation to the CGC in the presence of antagonists: cinanserin for 5-HT(2) and/or MDL72222 for 5-HT(3). The compound EPSP amplitude was partially suppressed by the application of these antagonists. The rise time of the compound EPSP was longer in the presence of MDL72222 than in that of cinanserin. These results suggest that these two subtypes of serotonin receptors are involved in the CGC-B1 synapses, and that these receptors contribute to compound EPSP. That is, the fast component of compound EPSP is mediated by 5-HT(3)-like receptors, and the slow component is generated via 5-HT(2)-like receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Central Nervous System / cytology
  • Central Nervous System / drug effects
  • Central Nervous System / physiology
  • Cinanserin / pharmacology
  • Feeding Behavior / physiology*
  • Neurons / drug effects
  • Neurons / physiology*
  • Pertussis Toxin / pharmacology
  • Receptors, Serotonin / classification*
  • Receptors, Serotonin / physiology*
  • Serotonin Antagonists / pharmacology
  • Snails / physiology*
  • Tropanes / pharmacology

Substances

  • Receptors, Serotonin
  • Serotonin Antagonists
  • Tropanes
  • Pertussis Toxin
  • Cinanserin
  • bemesetron