An RNA G-quadruplex in the 3' UTR of the proto-oncogene PIM1 represses translation

RNA Biol. Sep-Oct 2011;8(5):802-5. doi: 10.4161/rna.8.5.16038. Epub 2011 Jul 19.

Abstract

We have identified a conserved G-quadruplex forming sequence in the 3' untranslated region (UTR) of the proto-oncogene PIM1. Circular dichroism and thermal denaturation studies revealed that the PIM1 mRNA guanine-rich sequence forms a highly stable intramolecular G-quadruplex structure. Reporter gene assay demonstrated that the PIM1 RNA G-quadruplex represses translation. It is the first experimental evidence of an RNA G-quadruplex structure located in the 3' UTR that may act as posttranscriptional regulator.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Cell Line, Tumor
  • Circular Dichroism
  • Fungal Proteins
  • G-Quadruplexes*
  • Genes, Reporter
  • HeLa Cells
  • Humans
  • Mitogen-Activated Protein Kinases / genetics*
  • Nucleic Acid Conformation
  • Protein Biosynthesis
  • RNA Processing, Post-Transcriptional*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • 3' Untranslated Regions
  • Fungal Proteins
  • RNA, Messenger
  • Mitogen-Activated Protein Kinases
  • Pim1 protein, Pichia pastoris