Inhibition of deoxyribonucleic acid polymerases from human cells and from simian sarcoma virus by pyran

Biochem J. 1978 Nov 1;175(2):519-24. doi: 10.1042/bj1750519.


Pyrans are co-polymers of divinyl ether and maleic anhydride. Four pyrans of various molecular weights more potently inhibited terminal deoxyribonucleotidyltransferase (EC from a human cell line of acute lymphoblastic leukemia origin (Molt-4) than they did DNA polymerases alpha, beta and gamma from these cells and DNA polymerase from simian sarcoma virus. For example, the concentrations of one pyran required for 50% inhibition of terminal deoxynucleotidyltransferase, DNA polymerases alpha, beta and gamma and viral DNA polymerase were 0.9, 110, 125, 35 and 47 microgram/ml respectively. Quantitatively similar results were obtained with the other pyrans. Inhibition of these enzymes by pyran was dependent on the concentrations of both the bivalent cation and template/primer or initiator in assay mixtures, but not on the concentrations of the substrate (deoxyribonucleoside 5'-triphosphate), enzyme, or bovine serum albumin. These results suggested that pyran inhibited these enzymes by complexing bivalent cations, which caused a decreased affinity of template/primer or initiator for each enzyme and a decrease in enzyme activity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line
  • DNA Nucleotidylexotransferase / antagonists & inhibitors
  • Humans
  • Kinetics
  • Nucleic Acid Synthesis Inhibitors*
  • Polymers / pharmacology*
  • Pyran Copolymer / pharmacology*
  • Sarcoma Virus, Woolly Monkey / enzymology


  • Nucleic Acid Synthesis Inhibitors
  • Polymers
  • Pyran Copolymer
  • DNA Nucleotidylexotransferase