Elimination of mycoplasmal plate agglutination cross-reactions in sera from chickens inoculated with infectious bursal disease viruses

Avian Dis. 1990 Jul-Sep;34(3):663-7.


Sera from chickens inoculated with various challenge infectious bursal disease viruses or infectious bursal disease vaccines were found to cross-react in the Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) serum plate agglutination (SPA) tests. Two-fold dilutions of these cross-reacting sera with phosphate-buffered saline before retesting eliminated all non-specific agglutination in the MG and MS SPA tests. Cross-reactions were observed in the SPA test using sera from chickens inoculated with either MG or MS. Dilutions of these sera 1:2 had little effect on the number of these cross-reactions. At 1:4 serum dilutions, however, the number of cross-reactions between MG and MS was reduced. At 1:8 dilution of test sera, cross-reactions between MG and MS were further reduced. Some reduction in specific MG and MS SPA reactions, however, also occurred at the 1:8 dilution of sera with some of the plate antigen.

MeSH terms

  • Agglutination Tests / veterinary
  • Animals
  • Antibodies, Bacterial / immunology
  • Antibodies, Viral / immunology
  • Chickens*
  • Cross Reactions
  • False Positive Reactions
  • Immune Sera / immunology
  • Infectious bursal disease virus / immunology*
  • Mycoplasma / immunology*
  • Poultry Diseases / immunology*
  • Reoviridae Infections / immunology
  • Reoviridae Infections / veterinary*
  • Specific Pathogen-Free Organisms
  • Viral Vaccines / immunology


  • Antibodies, Bacterial
  • Antibodies, Viral
  • Immune Sera
  • Viral Vaccines