Maternal and zygotic requirements for src64 during Drosophila cellularization

Genesis. 2011 Dec;49(12):912-8. doi: 10.1002/dvg.20783. Epub 2011 Aug 5.

Abstract

Cellularization of the multinucleate Drosophila embryo occurs shortly after zygotic transcription begins. During cellular blastoderm morphogenesis, the microfilament cytoskeleton undergoes extensive reorganization. This cytoskeletal reorganization includes synchronized microfilament contraction regulated by src64, a gene encoding a Src nonreceptor tyrosine kinase. We report that src64 is maternally expressed in the Drosophila embryo and acts primarily as a maternal gene during cellularization. However, we show that src64 has some zygotic activity during late cellularization. By using compound chromosomes to generate embryos with wild-type levels of maternal src64 activity, we show that this zygotic activity is normally nonessential. We also report the identification of an alternate src64 transcript. Expression of this transcript is not affected by the src64Δ17 deletion mutation, explaining the presence of low levels of src64 activity observed in src64Δ17 mutants.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin Cytoskeleton / genetics*
  • Actin Cytoskeleton / metabolism
  • Alleles
  • Animals
  • Blastoderm / embryology
  • Blastoderm / metabolism*
  • Crosses, Genetic
  • Drosophila / embryology*
  • Drosophila / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Male
  • Molecular Sequence Data
  • Morphogenesis / genetics
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Drosophila Proteins
  • Proto-Oncogene Proteins
  • Protein-Tyrosine Kinases
  • Src64B protein, Drosophila

Associated data

  • GENBANK/GQ421601