Development of Glioblastoma Cell Lines Expressing Red Fluorescence for Non-Invasive Live Imaging of Intracranial Tumors

Anticancer Res. 2011 Jun;31(6):2161-71.

Abstract

Glioblastoma (GBM) cell lines expressing red fluorescent proteins were evaluated as a tool for non-invasive imaging of orthotopic tumors.

Materials and methods: mKate2- and mCherry-transduced U251MG GBM lines were sorted by flow cytometry. The growth rates and drug sensitivity of the resulting cell lines were compared to those of the parental line. Following orthotopic implantation, mKate2-expressing cells were detected using multispectral imaging.

Results: Flow cytometry-sorted fluorescent populations exhibiting growth curves that were comparable to those of the parental line were selected. mKate2-expressing cells were inoculated orthotopically and formed tumors which were visualized non-invasively, allowing monitoring of tumor growth over time and the assessment of tumor response to temozolomide drug treatment.

Conclusion: The strategy reported here led to the successful development of GBM models expressing mKate2 or mCherry. The fluorescence signal intensity measured in the brain of live animals correlates with tumor size, thus providing a method to assess tumor progression and response to treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Neoplasms / drug therapy
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Cell Line, Tumor
  • Drug Screening Assays, Antitumor
  • Female
  • Flow Cytometry
  • Glioblastoma / drug therapy
  • Glioblastoma / genetics
  • Glioblastoma / metabolism*
  • Glioblastoma / pathology
  • HEK293 Cells
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Luminescent Proteins / biosynthesis*
  • Luminescent Proteins / genetics
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID

Substances

  • Luminescent Proteins
  • red fluorescent protein