Antiproliferation and redifferentiation in thyroid cancer cell lines by polyphenol phytochemicals

J Korean Med Sci. 2011 Jul;26(7):893-9. doi: 10.3346/jkms.2011.26.7.893. Epub 2011 Jun 20.


Thyroid carcinogenesis is accompanied by loss of thyroid-specific functions and refractory to radioiodine and thyroid stimulating hormone (TSH) suppression therapy. Redifferentiating agents have been shown to inhibit tumor growth and improve the response to conventional therapy. Polyphenol phytochemicals (PPs) in fruits and vegetables have been reported to inhibit cancer initiation, promotion, progression and induce redifferentiation in selected types. In this study we examined PPs induce redifferentiation in thyroid cancer cell lines. We investigated the effects of genistein, resveratrol, quercetin, kaempferol, and resorcinol on the F9 embryonal carcinoma cell differentiation model. The thyroid cancer cell lines, TPC-1, FTC-133, NPA, FRO, and ARO, displayed growth inhibition in response to genistein, resveratrol, quercetin. We further demonstrated that genistein decreased the dedifferention marker CD97 in NPA cells and resveratrol decreased CD97 in FTC-133, NPA, FRO cells and quercetin decreased CD97 in all cell lines. We observed increased expression of differentiation marker NIS in FTC-133 cells in response to genistein, and resveratrol but no change in NPA, FRO, ARO cells. Quercetin increased or induced NIS in FTC-133, NPA, FRO cells. These findings suggest that PPs may provide a useful therapeutic intervention in thyroid cancer redifferentiation therapy.

Keywords: CD97; F9 Embryonal Carcinoma Cell; Phytochemical; Polyphenols; Redifferentiation; Sodium-Iodine Symporter; Thyroid Neoplasms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Carcinoma, Embryonal / drug therapy*
  • Carcinoma, Embryonal / metabolism
  • Cell Differentiation / drug effects*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Flavonoids / pharmacology*
  • Flavonoids / therapeutic use
  • Gene Expression Regulation, Neoplastic
  • Genistein / pharmacology
  • Genistein / therapeutic use
  • Humans
  • Kaempferols / pharmacology
  • Kaempferols / therapeutic use
  • Models, Biological
  • Phenols / pharmacology*
  • Phenols / therapeutic use
  • Polyphenols
  • Quercetin / pharmacology
  • Quercetin / therapeutic use
  • Receptors, G-Protein-Coupled
  • Resorcinols / pharmacology
  • Resorcinols / therapeutic use
  • Resveratrol
  • Stilbenes / pharmacology
  • Stilbenes / therapeutic use
  • Symporters / metabolism
  • Thyroid Neoplasms / drug therapy*
  • Thyroid Neoplasms / metabolism


  • ADGRE5 protein, human
  • Antigens, CD
  • Antineoplastic Agents
  • Flavonoids
  • Kaempferols
  • Phenols
  • Polyphenols
  • Receptors, G-Protein-Coupled
  • Resorcinols
  • Stilbenes
  • Symporters
  • sodium-iodide symporter
  • kaempferol
  • Quercetin
  • Genistein
  • Resveratrol
  • resorcinol