Abstract
A chemoenzymatic method was developed for the synthesis of macrocyclic peptides and glycopeptides. Sortase A was found to mediate either head to tail cyclization or oligomerization and then head to tail cyclization of peptides and glycopeptides, depending on the peptide length, to produce 15-mer or higher cyclic peptides and glycopeptides.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Aminoacyltransferases / metabolism*
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Bacterial Proteins / metabolism*
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Cyclization
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Cysteine Endopeptidases / metabolism*
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Glycopeptides / chemical synthesis*
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Glycopeptides / metabolism
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Gram-Positive Bacteria / enzymology*
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Molecular Sequence Data
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Peptides, Cyclic / chemical synthesis*
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Peptides, Cyclic / metabolism
Substances
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Bacterial Proteins
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Glycopeptides
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Peptides, Cyclic
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Aminoacyltransferases
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sortase A
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Cysteine Endopeptidases