Comparison of neoeriocitrin and naringin on proliferation and osteogenic differentiation in MC3T3-E1

Phytomedicine. 2011 Aug 15;18(11):985-9. doi: 10.1016/j.phymed.2011.03.002. Epub 2011 Jul 7.


Naringin is considered the main effective compound of Drynaria Rhizome, which is used commonly in the treatment of osteoporosis in traditional Chinese medicine. However, we found neoeriocitrin, a new compound isolated from Drynaria Rhizome, showed a better activity than naringin on proliferation and osteogenic differentiation in MC3T3-E1. Both neoeriocitrin and naringin exhibited the best effect on proliferation and osteogenic differentiation at concentration of 2μg/ml. Neoeriocitrin more significantly improved proliferation and alkaline phosphatase (ALP) activity as well as up-regulated Runx2, COLI and OCN expression by 56%, 37% and 14% respectively than naringin. Furthermore, neoeriocitrin could rescue the inhibition effect of cell differentiation induced by PD98059 to some degree. Therefore, neoeriocitrin may be a new promising candidate drug for treatment of osteoporosis.

Publication types

  • Comparative Study

MeSH terms

  • 3T3 Cells
  • Alkaline Phosphatase / metabolism
  • Animals
  • Cell Differentiation*
  • Cell Line
  • Cell Proliferation*
  • Disaccharides / pharmacology*
  • Dose-Response Relationship, Drug
  • Flavanones / pharmacology*
  • Flavonoids / pharmacology
  • Gene Expression
  • Mice
  • Osteoblasts / drug effects*
  • Osteogenesis / drug effects*
  • Polypodiaceae / chemistry
  • RNA, Messenger / metabolism


  • Disaccharides
  • Flavanones
  • Flavonoids
  • RNA, Messenger
  • neoeriocitrin
  • Alkaline Phosphatase
  • naringin
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one