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, 8 (8), 655-8

Large-scale Phosphosite Quantification in Tissues by a Spike-In SILAC Method

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Large-scale Phosphosite Quantification in Tissues by a Spike-In SILAC Method

Mara Monetti et al. Nat Methods.

Abstract

Despite progress in mass spectrometry (MS)-based phosphoproteomics, large-scale in vivo analyses remain challenging. Here we report a 'spike-in' stable-isotope labeling with amino acids in cell culture (SILAC) methodology using standards derived from labeled mouse liver cell lines, using which we analyzed insulin signaling. With this approach we identified 15,000 phosphosites and quantitatively compared 10,000 sites in response to insulin treatment, creating a very large, accurately quantified in vivo phosphoproteome dataset.

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References

    1. Nat Rev Mol Cell Biol. 2006 Feb;7(2):85-96 - PubMed
    1. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1488-93 - PubMed
    1. Cell. 2006 Nov 3;127(3):635-48 - PubMed
    1. Cell. 2008 Jul 25;134(2):353-64 - PubMed
    1. J Mass Spectrom. 2009 Jun;44(6):861-78 - PubMed

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