Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins

M Frey; J Reinecke; S Grant; H Saedler; A Gierl

doi:10.1002/j.1460-2075.1990.tb07625.x

Excision of the En/Spm transposable element of Zea mays requires two element-encoded proteins

EMBO J. 1990 Dec;9(12):4037-44. doi: 10.1002/j.1460-2075.1990.tb07625.x.

Authors

M Frey¹, J Reinecke, S Grant, H Saedler, A Gierl

Affiliation

¹ Max-Planck-Institut für Züchtungsforschung, Molekulare Pflanzengenetik, Köln, FRG.

Abstract

An excision assay system for En/Spm was developed in transgenic tobacco. The characteristics of excision and integration are similar to the natural system of Zea mays. In this transgenic model system two En/Spm encoded trans-acting functions, TNPA and TNPD, are required for excision. A biochemical model for transposition is proposed that might also be applicable to other transposable elements.

MeSH terms

Cloning, Molecular
Crosses, Genetic
DNA Transposable Elements*
DNA-Binding Proteins*
Molecular Sequence Data
Nicotiana / genetics
Oligonucleotide Probes
Phenotype
Plant Proteins / genetics*
Plants, Toxic
Plasmids
Polymerase Chain Reaction
Promoter Regions, Genetic
Repressor Proteins*
Restriction Mapping
Transcription, Genetic
Zea mays / genetics*

Substances

DNA Transposable Elements
DNA-Binding Proteins
Oligonucleotide Probes
Plant Proteins
Repressor Proteins