Poly(I:C) induce bone marrow precursor cells into myeloid-derived suppressor cells

Mol Cell Biochem. 2011 Dec;358(1-2):317-23. doi: 10.1007/s11010-011-0982-3. Epub 2011 Jul 9.


Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MDSC with LPS plus IFN-γ inhibited the DC development but enhanced MDSC functions, such as NO release and T cell suppression. In our study, bone marrow precursor cells cultures in GM-CSF and IL-4 were treated with poly(I:C) through the culture, Gr1(+)CD11b(+) cells with MDSC functions, such as NO release and T cell suppression were accumulated in the culture system. Then the similar phenomenon was observed in the vesicular stomatitis virus infection in vivo. In conclusion, we demonstrated that the bone marrow precursor cells in the presence of GM-CSF and IL-4 can differentiate into MDSC, which is dependent on the dynamic of interaction with poly(I:C).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / drug effects*
  • Bone Marrow Cells / metabolism
  • CD11b Antigen / metabolism
  • Cell Proliferation / drug effects
  • Cytokines / metabolism
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myeloid Cells / cytology*
  • Myeloid Cells / drug effects
  • Myeloid Cells / metabolism
  • Phagocytosis / drug effects
  • Phenotype
  • Poly I-C / pharmacology*
  • Rhabdoviridae Infections / immunology
  • Rhabdoviridae Infections / virology
  • Stem Cells / cytology*
  • Stem Cells / drug effects
  • Stem Cells / metabolism
  • T-Lymphocytes / cytology
  • T-Lymphocytes / drug effects
  • Vesiculovirus / physiology


  • CD11b Antigen
  • Cytokines
  • Poly I-C