In vitro activity of cefepime, a new parenteral cephalosporin, against recent European blood isolates and in comparison with piperacillin/tazobactam

Chemotherapy. 1990;36(4):259-67. doi: 10.1159/000238776.


Cefepime, a new parenteral cephalosporin with broad antibacterial spectrum and stability to the hydrolysis to many bacterial beta-lactamases, was tested against recent blood culture isolates (369 strains of gram-negative bacilli and 131 strains of staphylococci) collected in 29 European laboratories by the microdilution method in Mueller-Hinton broth. Cefepime was very active against the gram-negative bacilli (MIC50 less than or equal to 0.016-0.064 mg/l; MIC90 0.064-4 mg/l) and less active against Pseudomonas (MIC50 4 mg/l; MIC90 greater than 16 mg/l) or Acinetobacter (MIC50 and MIC90 greater than 16 mg/l). The staphylococci were also inhibited (MIC50 8 mg/l; MIC90 16 mg/l). Cefepime was very active against bacteria producing different plasmid-encoded beta-lactamases (MIC 0.016-0.5 mg/l). Piperacillin was not active against the latter strains (MIC from 2 to greater than 64 mg/l), but the presence of the beta-lactamase inhibitor tazobactam restored the activity of piperacillin. The bactericidal activity of cefepime and piperacillin/tazobactam against beta-lactamase-producing strains was confirmed by the killing curve technique.

Publication types

  • Comparative Study

MeSH terms

  • Cefepime
  • Cephalosporins / metabolism
  • Cephalosporins / pharmacology*
  • Drug Resistance, Microbial
  • Drug Synergism
  • Enterobacteriaceae / drug effects*
  • Enterobacteriaceae / enzymology
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / enzymology
  • Microbial Sensitivity Tests
  • Penicillanic Acid / metabolism
  • Penicillanic Acid / pharmacology*
  • Penicillin Resistance
  • Piperacillin / metabolism
  • Piperacillin / pharmacology*
  • Staphylococcus / drug effects*
  • Staphylococcus / enzymology
  • Tazobactam
  • beta-Lactamase Inhibitors
  • beta-Lactamases / metabolism*


  • Cephalosporins
  • beta-Lactamase Inhibitors
  • Cefepime
  • Penicillanic Acid
  • beta-Lactamases
  • Tazobactam
  • Piperacillin