Tn10-derived transposons active in Bacillus subtilis

J Bacteriol. 1990 Dec;172(12):6736-40. doi: 10.1128/jb.172.12.6736-6740.1990.

Abstract

Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / genetics*
  • Base Sequence
  • Blotting, Southern
  • DNA Transposable Elements*
  • DNA, Bacterial / genetics
  • Molecular Sequence Data
  • Plasmids
  • Recombination, Genetic
  • Restriction Mapping

Substances

  • DNA Transposable Elements
  • DNA, Bacterial