Acetaldehyde-collagen adducts in CCl4-induced liver injury in rats

Biochem Biophys Res Commun. 1990 Nov 30;173(1):111-9. doi: 10.1016/s0006-291x(05)81029-0.

Abstract

Circulating AC levels as well as antibodies against AC-protein adducts are increased in non-alcoholic liver injury. To identify the adducts, we used rats with CCl4-induced cirrhosis. Liver subcellular fractions were analyzed by immunochemical staining of protein slot blots and of electrophoretically separated proteins, transferred to nitrocellulose, using AC-protein adduct-specific antibodies. One reactive protein of about 200 kD was detected in the liver soluble fraction and in the cytosol of isolated hepatocytes and, to a lesser extent in the liver microsomes of CCl4-treated rats; in control animals, this reactivity was much weaker. The immunopositive AC adduct co-migrated with the beta 1,2 dimer of rat collagen type I; it was sensitive to digestion by a highly purified collagenase and also reacted with anti-rat collagen type I-specific IgG. In addition, comparison of peptides of the CNBr-digested, immunoprecipitated AC adduct with those of rat collagen type I revealed a high degree of similarity. Thus, AC adduct formation occurs in liver injury of non-alcoholic origin, and a target protein appears to be related to collagen type I, most likely the procollagen precursor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetaldehyde / metabolism*
  • Animals
  • Carbon Tetrachloride Poisoning / metabolism*
  • Cells, Cultured
  • Collagen / isolation & purification
  • Collagen / metabolism*
  • Cyanogen Bromide
  • Liver / metabolism*
  • Microbial Collagenase / metabolism
  • Molecular Weight
  • Peptide Fragments / isolation & purification
  • Rats
  • Rats, Inbred Strains
  • Reference Values

Substances

  • Peptide Fragments
  • Collagen
  • Microbial Collagenase
  • Acetaldehyde
  • Cyanogen Bromide