Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 223 (1), 97-106

HSP12, a New Small Heat Shock Gene of Saccharomyces Cerevisiae: Analysis of Structure, Regulation and Function

Affiliations

HSP12, a New Small Heat Shock Gene of Saccharomyces Cerevisiae: Analysis of Structure, Regulation and Function

U M Praekelt et al. Mol Gen Genet.

Abstract

We have isolated a new small heat shock gene, HSP12, from Saccharomyces cerevisiae. It encodes a polypeptide of predicted Mr 12 kDa, with structural similarity to other small heat shock proteins. HSP12 gene expression is induced several hundred-fold by heat shock and on entry into stationary phase. HSP12 mRNA is undetectable during exponential growth in rich medium, but low levels are present when cells are grown in minimal medium. Analysis of HSP12 expression in mutants affected in cAMP-dependent protein phosphorylation suggests that the gene is regulated by cAMP as well as heat shock. A disruption of the HSP12 coding region results in the loss of an abundant 14.4 kDa protein present in heat shocked and stationary phase cells. It also leads to the induction of the heat shock response under conditions normally associated with low-level HSP12 expression. The HSP12 disruption has no observable effect on growth at various temperatures, nor on the ability to acquire thermotolerance.

Similar articles

See all similar articles

Cited by 59 articles

See all "Cited by" articles

References

    1. EMBO J. 1987 May;6(5):1429-39 - PubMed
    1. Gene. 1987;59(1):1-18 - PubMed
    1. Mol Cell Biol. 1986 Jul;6(7):2324-33 - PubMed
    1. EMBO J. 1989 Nov;8(11):3171-6 - PubMed
    1. J Mol Biol. 1983 Nov 15;170(4):827-42 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback