Ultrastructure and cytochemistry of the yolk syncytial layer in the alevin of trout (Salmo fario trutta L. and Salmo gairdneri R.) after hatching. II. The cytoplasmic zone

Cell Tissue Res. 1979 Jan 30;196(1):75-93. doi: 10.1007/BF00236349.

Abstract

In contrast to the vitellolysis zone which is involved in the degradation of the yolk, the cytoplasmic zone of the yolk syncytial layer, composed of many cytoplasmic organelles, is implicated in a secretory process. The granular endoplasmic reticulum of the latter is extremely well developed and organized into trabeculae. The yolk nuclei show a RNA positive reaction. The Golgi apparatus is implicated in the elaboration of very low density lipoproteins (VLDL) and of acid phosphatase. The numerous mitochondria seem to suggest an important energy metabolism in the solubilisation area. The appearance of certain special structures (crystalline bodies, pseudovesicular structures, lipochondria) as well as their relation with the organelles of the cytoplasmic zone, re-inforces the impression of a layer with a secretory nature and suggests its participation in the remodelling of the yolk products produced in the vitellolysis zone. The results of the investigations concerning the acid phosphatase activity suggest that this enzyme plays a role on the one hand in the degradation of certain platelets which penetrate into the cytoplasmic zone, and on the other hand in the regulation of VLDL, in the lysis of secretory products elaborated by the cytoplasmic zone. The possible presence of microperoxisomes is discussed as well as the positive detection of glucose-6-phosphate dehydrogenase.

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Cell Nucleus / ultrastructure
  • Cytoplasm / ultrastructure
  • Egg Yolk*
  • Endoplasmic Reticulum / ultrastructure
  • Female
  • Glucosephosphate Dehydrogenase / metabolism
  • Golgi Apparatus / ultrastructure
  • Inclusion Bodies / ultrastructure
  • Lipoproteins, VLDL
  • Mitochondria / ultrastructure
  • Organoids / ultrastructure
  • Peroxidases / metabolism
  • Salmonidae / physiology*
  • Thiamine Pyrophosphatase / metabolism
  • Trout / physiology*

Substances

  • Lipoproteins, VLDL
  • Glucosephosphate Dehydrogenase
  • Peroxidases
  • Acid Phosphatase
  • Thiamine Pyrophosphatase