Evidences for the existence of endothelium-derived relaxing factor in the renal medulla

Am J Hypertens. 1990 Nov;3(11):876-8. doi: 10.1093/ajh/3.11.876.

Abstract

The basal levels of cGMP in renal medulla slices were enhanced when the slices were stimulated with both endothelium-dependent (acetylcholine) and endothelium-independent (molsidomine) vasodilators. When preincubated with NG-mono-methyl-L-arginine, a specific inhibitor of endothelium-derived relaxing factor, only the acetylcholine-stimulated increase was completely abolished. Furthermore, a preincubation with L-arginine, a selective precursor of endothelium-derived relaxing factor, enhanced the cGMP levels. The results indicate that the renal medulla, presumably the endothelial cells of the vasa recta, is able to produce endothelium-derived relaxing factor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylcholine / pharmacology
  • Animals
  • Arginine / analogs & derivatives
  • Arginine / pharmacology
  • Cyclic GMP / analysis
  • Cyclic GMP / metabolism*
  • Dogs
  • Female
  • Kidney Medulla / chemistry
  • Kidney Medulla / drug effects
  • Kidney Medulla / metabolism*
  • Male
  • Molsidomine / pharmacology
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / metabolism*
  • Premedication
  • omega-N-Methylarginine

Substances

  • omega-N-Methylarginine
  • Nitric Oxide
  • Arginine
  • Molsidomine
  • Cyclic GMP
  • Acetylcholine