Quantitative plant phosphoproteomics

Curr Opin Plant Biol. 2011 Oct;14(5):507-11. doi: 10.1016/j.pbi.2011.06.008. Epub 2011 Jul 18.


Protein phosphorylation is a major post-translational modification in plants crucial for the regulation of diverse cellular functions. In the early stages of this field, efforts were focused on the qualitative detection, identification, and cataloging of in vivo protein phosphorylation sites. Recently these studies have advanced into utilizing quantitative mass spectrometric measurements, capable of dynamically monitoring changes in phosphorylation levels in response to genetic and environmental alterations. This review will highlight current untargeted and targeted mass spectral technologies used for quantitative phosphoproteome measurements in plants, and provide a discussion of these phosphorylation changes in relation to important biological events.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Phosphoproteins / metabolism
  • Phosphorylation
  • Plant Proteins / metabolism*
  • Plants / metabolism*
  • Proteome / metabolism
  • Proteomics / methods
  • Signal Transduction


  • Phosphoproteins
  • Plant Proteins
  • Proteome