Abstract
Studies investigating the subcellular localization of periplasmic proteins have been hampered by problems with the export of green fluorescent protein (GFP). Here we show that a superfolding variant of GFP (sfGFP) is fluorescent following Sec-mediated transport and works best when the cotranslational branch of the pathway is employed.
Copyright © 2011, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Green Fluorescent Proteins / analysis*
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Green Fluorescent Proteins / genetics*
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Molecular Biology / methods
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Periplasm / chemistry*
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Periplasmic Proteins / analysis*
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Periplasmic Proteins / genetics*
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Recombinant Fusion Proteins / analysis
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Recombinant Fusion Proteins / genetics
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Staining and Labeling / methods
Substances
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Periplasmic Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins